Cofilin 1 (CFL1) (+ Cofilin 2) Rabbit Polyclonal Antibody

CAT#: AP08086PU-S

Cofilin 1 (CFL1) (+ Cofilin 2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from MDA cells using cofilin1/cofilin2 antibody

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed HeLa cells using Cofilin-1/2 Antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 86~90(A-T-Y-E-T).
• Specificity: This antibody detects endogenous levels of total Cofilin-1/2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: cofilin 1
• Database Link:
  Entrez Gene 12631 MouseEntrez Gene 1072 Human
  UniProt ID P23528
• Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.
• Synonyms: CFL1, CFL, p18

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton