HDAC2 pSer394 Rabbit Polyclonal Antibody

CAT#: AP08014PU-N

HDAC2 pSer394 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from HT-29 cells untreated or treated with UV (20min), using HDAC2 antibody (Lane 1 and 2) and HDAC2 (pSer394) antibody (Lane 3 and 4).

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  Figure 4 Western Blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2 (pSer394) antibody

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HDAC2 (pSer394) antibody.

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  Figure 3 Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (pSer394) antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).
• Specificity: This antibody HDAC2 (pSer394) detects endogenous levels of HDAC2 only when phosphorylated at Serine 394.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: histone deacetylase 2
• Database Link:
  Entrez Gene 3066 Human
  UniProt ID Q92769
• Background: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
• Synonyms: Histone deacetylase 2, HD2