p53 (TP53) pSer6 Rabbit Polyclonal Antibody

CAT#: AP08001PU-N

p53 (TP53) pSer6 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extract from HT29 and Hela cells untreated or treated with UV using p53 antibody (phospho-Ser6)

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho-Ser6) antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500~1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50~1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Phosphopeptide derived from Human p53 around the phosphorylation site of Serine 6 (P-Q-Sp-D-P).
• Specificity: This antibody detects endogenous levels of p53 only when phosphorylated at Serine 6.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific peptide: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
• Note: Molecuar Weight: 53 kDa