EZH1 Rabbit Polyclonal Antibody
EZH1 rabbit polyclonal antibody, Aff - Purified
|Applications||IF, IHC, WB|
|Recommended Dilution||Western blot: 1/500-1/1000.
Immunohistochemistry obn Paraffin Sections: 1/50-1/200.
|Immunogen||Synthetic peptide, corresponding to amino acids 162-208 of Human ENX-2.|
|Specificity||This antibody detects endogenous levels of ENX-2 protein.
(region surrounding Glu192)
|Formulation||Phosphate buffered saline (PBS), pH~7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Preservative: 0.05% Sodium Azide
|Purification||Affinity Chromatography using epitope-specific immunogen.|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Predicted Protein Size||~85 kDa|
|Gene Name||Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1)|
|Background||In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG) proteins, which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. ENX-2 is another mammalian homolog of the Drosophila gene enhancer of zeste and contains one SET domain. The gene for human ENX-2 maps to chromosome 17q21. ENX-2 expression is ubiquitous in adult and fetal tissue, where it may aid in maintaining heterochromatin stability.|
|Protein Families||Druggable Genome, Transcription Factors|
|The use of this Antibodies has been cited in the following citations:|
The linear ubiquitin assembly complex (LUBAC) is essential for NLRP3 inflammasome activation
,Rodgers MA, Bowman JW, Fujita H, Orazio N, Shi M, Liang Q, Amatya R, Kelly TJ, Iwai K, Ting J, Jung JU,
J. Exp. Med. 2014
,PubMed ID 24958845
mTor is a signaling hub in cell survival: a mass-spectrometry-based proteomics investigation
,Tang Z, Baykal AT, Gao H, Quezada HC, Zhang H, Bereczki E, Serhatli M, Baykal B, Acioglu C, Wang S, Ioja E, Ji X, Zhang Y, Guan Z, Winblad B, Pei JJ,
J. Proteome Res. 2014
,PubMed ID 24694195