Thrombin Receptor (F2R) Rabbit Polyclonal Antibody
Thrombin Receptor (F2R) rabbit polyclonal antibody, Aff - Purified
|Recommended Dilution||Western blot: 1/500-1/1000.|
|Immunogen||Synthetic peptide, corresponding to the N-terminal of Human Thrombin R.|
|Specificity||This antibody detects endogenous levels of PAR1 protein.
(region surrounding Gly17)
|Formulation||Phosphate buffered saline (PBS), pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Preservative: 0.05% Sodium Azide
|Purification||Affinity Chromatography using epitope-specific immunogen.|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Predicted Protein Size||~ 48 kDa|
|Gene Name||coagulation factor II thrombin receptor|
|Background||Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor. Cytokines including TNF-alpha and IL-1beta increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.|
|Synonyms||Proteinase-activated receptor 1, PAR-1, PAR1, CF2R|