Casein Kinase 2 beta (CSNK2B) Rabbit Polyclonal Antibody

CAT#: AP06515PU-N

Casein Kinase 2 beta (CSNK2B) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Casein Kinase IIβ antibody at 1/500 dilution Lane 1:Jukat whole cell lysate Lane 2:Hela whole cell lysate Lane 3:Mouse brain tissue lysate Lane 4:Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of CK2-β antibody in paraffin-embedded human breast tissue

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 175-220 of Human Casein Kinase IIβ.
• Specificity: This antibody antibody detects endogenous levels of CK2-beta protein.
  (region surrounding Ser205)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: casein kinase 2 beta
• Database Link:
  Entrez Gene 1460 Human
  UniProt ID P67870
• Background: Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, γ, ε and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The α catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.
• Synonyms: CK II beta, Phosvitin, G5a, CSNK2B, CK2N