BCL2 Rabbit Polyclonal Antibody
BCL2 rabbit polyclonal antibody, Aff - Purified
|Applications||IF, IHC, WB|
|Recommended Dilution||Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
|Reactivity||Human, Mouse, Rat|
|Immunogen||Synthetic peptide, corresponding to amino acids 31-80 of Human Bcl-2.|
|Specificity||This antibody detects endogenous levels of BCL-2 protein.
(region surrounding Pro65)
|Formulation||Phosphate buffered saline (PBS), pH 7.2.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 15 mM Sodium Azide
|Purification||Affinity Chromatography using epitope-specific immunogen|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Predicted Protein Size||~ 26 kDa|
|Background||Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.|
|Synonyms||BCL2, Bcl-2 alpha|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.