5 Lipoxygenase (ALOX5) Rabbit Polyclonal Antibody

CAT#: AP06493PU-N

5 Lipoxygenase (ALOX5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ALOX5 antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Rat lung tissue lysate

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  Immunohistochemistry analyzes of ALOX5 antibody in paraffin-embedded human skeletal muscle tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 231-280 of Human ALOX5.
• Specificity: This antibody detects endogenous levels of ALOX5 protein.
  (region surrounding Glu267)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~78 kDa
• Gene Name: arachidonate 5-lipoxygenase
• Database Link:
  Entrez Gene 240 Human
  UniProt ID P09917
• Background: 5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells. 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis. The cellular localization of 5-LO varies between cell types. In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope. In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane. This intracellular shuttling of 5-LO is dependent on the associationwith various signaling molecules, phosphorylation and the presence of a distinct nuclear localization signal, which is encoded at the amino terminus of 5-LO.
• Synonyms: 5-lipoxygenase, 5-LO, Arachidonate 5-lipoxygenase