Ubiquitin (UBB) Rabbit Polyclonal Antibody

CAT#: AP06474PU-N

Ubiquitin (UBB) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Ubiquitin Antibody at 1/500 dilution: Lane 1: Hela cell lysate treated with UV. Lane 2: HEK293T cell ysate Lane 3: NIH-3T3 cell lysate treated with UV. Lane 4: Rat kidney tissue lysate.

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  Immunohistochemistry analysis of Ubiquitin Antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 11-60 of Human Ubiquitin.
• Specificity: This antibody detects endogenous levels of Ubiquitin protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~8.5 kDa
• Gene Name: ubiquitin B
• Database Link:
  Entrez Gene 7314 Human
  UniProt ID P0CG47
• Background: Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IkappaB, p53, cdc25a and Bcl-2 have been shown to be targets for the ubiquitination-proteosome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis.
• Synonyms: UBB, Polyubiquitin-B