BCAR1 Rabbit Polyclonal Antibody

CAT#: AP06404PU-N

BCAR1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p130 Cas antibody in extracts from NIH/3T3 cells.

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  Immunohistochemistry (IHC) analyzes of p130 Cas antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 380-430 of Human p130 Cas.
• Specificity: This antibody detects endogenous levels of p130 Cas protein.
  (region surrounding Val404)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 130 kDa
• Gene Name: BCAR1, Cas family scaffolding protein
• Database Link:
  Entrez Gene 9564 Human
  UniProt ID P56945
• Background: The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
• Synonyms: p130cas, CASS1