p15 INK4b (CDKN2B) Rabbit Polyclonal Antibody
p15 INK4b (CDKN2B) rabbit polyclonal antibody, Aff - Purified
|Applications||IF, IHC, WB|
|Recommended Dilution||Western blot: 1/500 - 1/1000.
Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Immunofluorescence: 1/50 - 1/200.
|Reactivity||Human, Mouse, Rat|
|Immunogen||Synthetic peptide, corresponding to amino acids 100-150 of Human p15 INK4b.|
|Specificity||This antibody detects endogenous levels of p15 INK protein.
(region surrounding Gly113)
|Formulation||Phosphate buffered saline (PBS), pH~7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure SDS-PAGE).
Preservative: 0.05% Sodium Azide
|Purification||Affinity Chromatography using epitope-specific immunogen|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: One year from despatch.|
|Predicted Protein Size||~ 15 kDa|
|Gene Name||Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1|
|Background||The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFβ-treated human keratinocytes, suggesting that p15 may act as an effector of TGFβ-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFβ-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.|
|Synonyms||p14INK4b, MTS2, p14-INK4b, p15-INK4b|
|Protein Families||Druggable Genome|
|Protein Pathways||Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway|
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