GABA A Receptor beta 1 (GABRB1) Rabbit Polyclonal Antibody

CAT#: AP06129PU-N

GABA A Receptor beta 1 (GABRB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of GABAA Rβ1 antibody at 1/500 dilution Lane 1: MCF-7 cell lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate

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  Immunohistochemistry (IHC) analysis of GABA-RB antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human GABAA Rβ1.
• Specificity: This antibody detects endogenous levels of GABA-RB protein.
  (region surrounding Arg328)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 54 kDa
• Gene Name: gamma-aminobutyric acid type A receptor beta1 subunit
• Database Link:
  Entrez Gene 2560 Human
  UniProt ID P18505
• Background: GAD-65 and GAD-67, glutamate decarboxylases function to catalyze the production of GABA (γ-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rα1-6, GABAA Rβ1-3, GABAA Rε, GABAA Rδ, GABAA Rγ1-3, GABAA Rγ1 and GABAA Rγ2. The GABAB family is composed of GABAB R1α and GABAB R1β. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2 and -3). The GABA transporters function to terminate GABA action.
• Synonyms: GABRB-1, GABA A receptor subunit beta-1