HA Epitope Tag (YPYDVPDYA) Rabbit Polyclonal Antibody

SKU AP05576PU-N

HA Epitope Tag (YPYDVPDYA) rabbit polyclonal antibody, Purified

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Citrate buffer is recommended for this purpose).; ELISA.; Western blot.
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
• Specificity: This antibody is specific for the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors.; The antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
• Buffer: 0.02M Potassium phosphate, 0.15M Sodium chloride pH7.2, 0.01% Sodium Azide (NaN3); State: Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Reference Data