Smoothened (SMO) Rabbit Polyclonal Antibody

CAT#: AP05293PU-N

Smoothened (SMO) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using SMO antibody on human brain lysate. Lysate loaded at 15 ug/lane. Antibody used at 10 ug/ml (1) and 5 ug/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot (1:400 starting dilution).
  Positive control: Brain.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from the human SMO protein
• Specificity: This antibody detects SMO (Smoothened Homolog).
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: smoothened, frizzled class receptor
• Database Link:
  Entrez Gene 6608 Human
  UniProt ID Q99835
• Background: SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.
• Synonyms: SMOH, Smoothened homolog

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer