Sphk1 Rabbit Polyclonal Antibody

CAT#: AP05256PU-N

Sphk1 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:400 starting dilution.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
• Specificity: This antibody detetcs Sphingosine Kinase 1.
• Formulation: PBS with 0.08 % sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sphingosine kinase 1
• Database Link:
  Entrez Gene 20698 Mouse
  UniProt ID Q8CI15
• Background: Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.
• Synonyms: SPHK, SPK, SPK 1, SPK-1