AKAP12 / SSeCKS Sheep Polyclonal Antibody

CAT#: AP05065PU-N

AKAP12 / SSeCKS sheep polyclonal antibody, Purified

Product Images

Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/5%NFDM, probed with 5 g/ml of pre-immune serum 5 g of SSeCKS antibod

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 2-5 µg/ml.
  Immunoprecipitation.
  Immunohistochemistry.
• Reactivities: Bovine, Rat
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
• Specificity: This antibody reacts to SSeCKS (Src-Suppressed C Kinase Substrate).
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation.
• Conjugation: Unconjugated
• Storage: The antibody can be shipped at ambient temperature.
  Store (in aliquots) at -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q62766
• Background: Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicatedduring the process of spermiogenesis and in the actinbased stellate morphology of mesangial cells.
• Note: Predicted Molecular Weight: 172.5 kDa