MARCKS Rabbit Polyclonal Antibody

CAT#: AP02758PU-N

MARCKS rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extract from HeLa cells, using MARCKS antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:500~1:1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of aa 160~164 (K-K-S-F-K).
• Specificity: MARCKS antibody detects endogenous levels of total MARCKS protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: myristoylated alanine rich protein kinase C substrate
• Database Link:
  Entrez Gene 4082 Human
  UniProt ID P29966
• Background: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
• Synonyms: Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL