GJA1 Rabbit Polyclonal Antibody

CAT#: AP02752PU-S

GJA1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HT29 and HepG2 cells using Connexin-43 antibody.

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  Immunohistochemistry analysis of Paraffin-Embedded Human Breast Carcinoma tissue using Connexin-43 antibody (Left) or the same antibody preincubated with blocking peptide (Right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic KLH conjugated Peptide sequence around amino acids 365~369 (R-A-S-S-R) from Human Connexin-43.
• Specificity: This antibody detects endogenous levels of total Connexin 43 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 50% Glycerol
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: gap junction protein alpha 1
• Database Link:
  Entrez Gene 2697 Human
  UniProt ID P17302
• Background: GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.
• Synonyms: Gap junction alpha-1 protein, Connexin-43, GJAL
• Note: Molecular Weight: 43 kDa