BCL2 Rabbit Polyclonal Antibody

CAT#: AP02601PU-S

BCL2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from MCF cells using BCL-2 antibody and the same antibody preincubated with blocking peptide

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 antibody.

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  Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
  Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L)
• Specificity: BCL-2 antibody detects endogenous levels of total BCL-2 protein.
• Formulation: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends.
  BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
• Synonyms: BCL2, Bcl-2 alpha