GSK3 beta (GSK3B) Rabbit Polyclonal Antibody

CAT#: AP02546PU-N

GSK3 beta (GSK3B) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from A2780 and HT29 cells using GSK3beta antibody

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  Western blot analysis of extracts from 293 cells treated with 10% serum (30min), using GSK3β antibody.

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3β antibody.

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  Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining using GSK3 beta antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic non-phosphopeptide derived from human GSK3-beta around the phosphorylation site of serine 9 (T-T-SP-F-A).
• Specificity: This antibody detects endogenous levels of total GSK3-beta protein and may cross-react with the GSK3-alpha due to high sequence homology.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  Entrez Gene 2932 Human
  UniProt ID P49841
• Background: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B