EGFR pTyr1172 Rabbit Polyclonal Antibody
Other products for "EGFR"
Specifications
Product Data | |
Applications | IHC, WB |
Recommended Dilution | Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50-1/100. |
Reactivities | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Peptide sequence around phosphorylation site of Tyrosine 1172 (P-D-Yp-Q-Q) derived from HUman EGFR. |
Specificity | This antibody detects endogenous levels EGFR only when phosphorylated at Tyrosine 1172. |
Formulation | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 50% Glycerol Preservative: 0.02% Sodium Azide |
Concentration | lot specific |
Purification | Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Conjugation | Unconjugated |
Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | epidermal growth factor receptor |
Database Link | |
Background | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. |
Synonyms | Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1 |
Reference Data |
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in shipping.