MEK1 (MAP2K1) pSer218/222 Rabbit Polyclonal Antibody

CAT#: AP02471PU-S

MEK1 (MAP2K1) pSer218/222 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HeLa cells untreated or treated with UV using MEK1/MEK2 antibody (pSer217/221)

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MEK1/MEK2 Antibody (pSer217/221) (left) or the same antibody preincubated with Blocking peptide (right)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:
  Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around the phosphorylation site of Serine 217/221 (I-D-Sp-M-A) derived from Human MEK1/MEK2
• Specificity: This antibody detects endogenous levels of MEK1/MEK2 only when phosphorylated at Serine 217/221.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45 kDa
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  Entrez Gene 5604 Human
  UniProt ID Q02750
• Synonyms: MAPKK1, ERK activator kinase 1, ERK activator kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MEK1, MEK2, PRKMK1, PRKMK2, MAP kinase kinase 1, MAP kinase kinase 2