AKT2 pSer474 Rabbit Polyclonal Antibody

CAT#: AP02412PU-S

AKT2 pSer474 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis using Akt2 (Ab-474) antibody (Lane 1, 2, 3 and 4) and Akt2 (phospho- Ser474) antibody (Lane 5, 6 and 7).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (phospho- Ser474) antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Suitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: AKT serine/threonine kinase 2
• Database Link:
  Entrez Gene 208 Human
  UniProt ID P31751
• Background: The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3.
  AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
• Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta