AKT1 pThr308 Rabbit Polyclonal Antibody

CAT#: AP02354PU-N

AKT1 pThr308 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from Hela cells untreated or treated with EGF serum or both using AKT (pThr308) Antibody

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (pThr308) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescene staining of methanol-fixed Hela cells showing nuclear dot staining using AKT (pThr308) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C).
• Specificity: Akt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  Entrez Gene 207 Human
  UniProt ID P31749
• Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT