AKT1 pSer473 Rabbit Polyclonal Antibody

CAT#: AP02353PU-S

AKT1 pSer473 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (phospho-Ser473) antibody.

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around the phosphorylation site of Ser473 (Q-F-Sp-Y-S) derived from Human Akt
• Specificity: This antibody detects endogenous levels of Akt only when phosphorylated at Serine 473.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  Entrez Gene 207 Human
  UniProt ID P31749
• Background: The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases.
  Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
• Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
• Note: Molecular Weight: 60 kDa