Nidogen-2 Rabbit Polyclonal Antibody

CAT#: AP02275SU-N

Nidogen-2 rabbit polyclonal antibody, Serum

Product Images

Immunofluorescence of Nidogen 2 staining in Paraffin sections of cardiac muscle tissue from control (+/+) and Nidogen 2-mutant (-/-) mice. Image B: In control sections, staining for Nidogen 2 was prominent around capillaries, but not around cardiomyocytes.

Specifications

Product Data

• Applications: ELISA, IHC, R, WB
• Recommended Dilution: RIA (See Schymeinsky, 2002).
  ELISA: 1/5000-1/16000 (See Kohfeldt, 1998, and Salmivirta, 2002).
  Western blot: 1/400-1/1000 (See Schymeinsky, 2002, and Kunze,2010)
  Immunohistochemistry on Paraffin Sections: 1/1000.
  Immunohistochemistry on Cryosections: 1/1000.
  For References on IHC See: Kohfeldt, 1998, Salmivirta, 2002, Schymeinsky, 2002, and Kunze,2010.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Mouse Nidogen 2
• Specificity: This antibody detects Mouse Nidogen-2. Other species not tested.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: nidogen 2
• Database Link:
  Entrez Gene 18074 Mouse
  UniProt ID O88322
• Background: Nidogen 2 is an adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell extracellular matrix interactions.
• Synonyms: NID-2,NID2
• Note: Locus ID: 18074