EIF4B pSer422 Rabbit Polyclonal Antibody

CAT#: AP01821PU-N

EIF4B pSer422 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of eIF4B pSer422 antibody (Cat.-No.: AP01821PU-N) in extracts from NIH/3T3 cells.

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  Immunohistochemistry (IHC) analyzes of eIF4B pSer422 antibody (Cat.-No.: AP01821PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of eIF4B pSer422 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: eukaryotic translation initiation factor 4B
• Database Link:
  Entrez Gene 1975 Human
  UniProt ID P23588
• Background: eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
• Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B