Chk2 (CHEK2) pThr68 Rabbit Polyclonal Antibody

CAT#: AP01556PU-N

Chk2 (CHEK2) pThr68 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot (WB) analysis of p-Chk2 antibody (pThr68) in extracts from NIH/3T3 cells treated with UV. (Cat.-No.: AP01556PU-N)

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  Figure 2. Immunohistochemistry (IHC) analyzes of p-Chk2 antibody (pThr68) in paraffin-embedded human breast carcinoma tissue. (Cat.-No.: AP01556PU-N)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Chk2 only when phosphorylated at Thr68.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 62 kDa
• Gene Name: checkpoint kinase 2
• Database Link:
  Entrez Gene 11200 Human
  UniProt ID O96017
• Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
• Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1