S1PR2 Rabbit Polyclonal Antibody
S1PR2 rabbit polyclonal antibody, Aff - Purified
|Applications||IF, IHC, WB|
|Recommended Dilution||Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
|Reactivities||Human, Mouse, Rat|
|Immunogen||Synthetic peptide, corresponding to amino acids 281-330 of Human EDG-5.|
|Specificity||This antibody detects endogenous levels of EDG-5 protein. (region surrounding Leu303)|
|Formulation||PBS, pH~7.2, 50% Glycerol
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 0.02% Sodium Azide
|Purification||Affinity Chromatography using epitope-specific immunogen|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: One year from despatch.|
|Predicted Protein Size||~ 39 kDa|
|Gene Name||sphingosine-1-phosphate receptor 2|
|Background||The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain. EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.|
|Synonyms||S1P receptor 2, S1PR2, EDG5, H218, LPB2, AGR16, Gpcr13|