CCN3 (NOV) Rabbit Polyclonal Antibody

CAT#: AP01154PU-N

CCN3 (NOV) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Indirect ELISA: To detect hNOV by (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antibody in conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV.
  Sandwich ELISA: To detect hNOV (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human NOV (AP01154BT-S or AP01154BT-N) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV.
  Western blot: To detect hNOV this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry on Formalin-Fixed, Paraffin Sections: 0.25-0.50 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
  Positive Control: Human breast invasive ductal carcinoma.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) recombinant Human NOV
• Specificity: This antibody detects NOV.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized (sterile filtered) Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the lyophilized antibody at -20°C.
  Following reconstitution it is stable for two weeks at
  2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: nephroblastoma overexpressed
• Database Link:
  Entrez Gene 4856 Human
  UniProt ID P48745
• Background: CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.
• Synonyms: CCN3, IGFBP9, NOVH, NovH
• Note: Centrifuge vial prior to opening.