Eotaxin 3 (CCL26) Goat Polyclonal Antibody

CAT#: AP01122PU-S

Eotaxin 3 (CCL26) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
  ELISA: Indirect: To detect hEotaxin-3 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
  Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human Eotaxin-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
  Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived, 8.4 kDa recombinant human Eotaxin-3
• Specificity: This antibody detects Eotaxin-3.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 10344 Human
  UniProt ID Q9Y258
• Background: Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils.
• Synonyms: C-C motif chemokine 26, Small-inducible cytokine A26, SCYA26, MIP4 alpha, IMAC, TSC-1, TSC1