GST-Tag Goat Polyclonal Antibody

CAT#: AP01064BT-N

GST-Tag goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Schistosoma japonicum
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified Glutathione-STransferase [Schistosoma japonicum].
  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: Biotin
  State: Lyophilized IgG fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P08515
• Background: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag