Mfn2 (370-600, Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: S153-5]
Mfn2 (370-600, Cytopl. Dom.) mouse monoclonal antibody, clone S153-5, Purified
Product Datasheet for 'AM60065PU-N'
|Applications||IF, IHC, WB|
|Recommend Dilution||Western blot: 1/1000; 1 μg/ml was sufficient for detection of Mitofusin-2 in 20 μg of mouse cardiac mitochondrial lysate by colorimetric immunoblot using HRP conjugated secondary antibody.
Immunohistochemistry:Free floating sections, fixed in formaldehyde.
|Immunogen||Fusion protein amino acids 370-600 (Cytoplasmic, N-terminus) of mouse Mitofusin-2 (Accession no. Q80U63); ~55% identity with Mitofusin-1.|
|Specificity||This antibody detects a 90 kDa protein. No cross-reactivity against Mitofusin-1.|
|Formulation||PBS pH 7.4, 50% Glycerol, 0,09% Sodium azide
State: Liquid purified IgG fraction
|Purification||Protein G chromatography|
|Background||Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression.|
|Synonyms||MFN2, CPRP1, KIAA0214|