DHFR / DHFRP1 Mouse Monoclonal Antibody [Clone ID: AT5B2]
DHFR / DHFRP1 mouse monoclonal antibody, clone AT5B2, Purified
|Recommended Dilution||The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.|
|Immunogen||Recombinant human DHFR (1-187aa) purified from E. coli.|
|Formulation||Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
State: Liquid purified Ig fraction
|Purification||Protein-A affinity chromatography|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1|
|Background||Dihydrofolate reductase (DHFR) catalyzes the DPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of D synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.|
|Protein Families||Druggable Genome, Stem cell - Pluripotency|
|Protein Pathways||Folate biosynthesis, Metabolic pathways, One carbon pool by folate|