GSTT1 Mouse Monoclonal Antibody [Clone ID: AT38D11]

CAT#: AM50631PU-N

GSTT1 mouse monoclonal antibody, clone AT38D11, Purified

Product Images

The recombinant human GSTT1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GSTT1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: Jurkat cell lysate Lane 5.: HL-60 cell lysate

Specifications

Product Data

• Clone Name: AT38D11
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human GSTT1 (1-240aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: glutathione S-transferase theta 1
• Database Link:
  Entrez Gene 2952 Human
  UniProt ID P30711
• Background: Glutathione S-transferase theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes (alpha, mu, pi, theta, and zeta). The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
• Synonyms: Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1

Reference Data

• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450