Peroxiredoxin-6 / PRDX6 Mouse Monoclonal Antibody [Clone ID: AT22E7]

CAT#: AM50622PU-N

Peroxiredoxin-6 / PRDX6 mouse monoclonal antibody, clone AT22E7, Purified

Product Images

The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant PRDX6 protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: U87MG cell lysate Lane 6.: Ramos cell lysate Lane 7.: Mouse brain lysate

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  ICC/IF analysis of PRDX6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT22E7
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human PRDX6 (1-224aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: peroxiredoxin 6
• Database Link:
  Entrez Gene 9588 Human
  UniProt ID P30041
• Background: Peroxiredoxin 6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctiol enzyme with two distinct active sites. It is involved in redox regulation of the cell. it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
• Synonyms: PRDX-6, Prx6, Prx-6, 1-Cys PRX, aiPLA2, NSGPx, AOP2, AOP-2, KIAA0106

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Methane metabolism, Phenylalanine metabolism