BCAT1 Mouse Monoclonal Antibody [Clone ID: AT3C8]

CAT#: AM50619PU-S

BCAT1 mouse monoclonal antibody, clone AT3C8, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BCAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : HeLa cell lysate

Specifications

Product Data

• Clone Name: AT3C8
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human BCAT1 (1-386aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: branched chain amino acid transaminase 1
• Database Link:
  Entrez Gene 586 Human
  UniProt ID P54687
• Background: BCAT1, also known as branched chain amino-acid transamise (cytosolic form), catalyzes the reversible transamition of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamition: hypervalinemia and hyperleucine-isoleucinemia. BCAT1 is expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
• Synonyms: BCT1, ECA39, BCAT(c)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation