RAIDD (CRADD) Mouse Monoclonal Antibody [Clone ID: AT14G8]

CAT#: AM50351PU-S

RAIDD (CRADD) mouse monoclonal antibody, clone AT14G8, Purified

Product Images

Western blot analysis: The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: The Recombinant Human CRADD (50, 100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT14G8
• Applications: ELISA, WB
• Recommended Dilution: This antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity.
  Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
  Recommended Starting Dilution: 1/500.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human CRADD (1-199aa) purified from E. coli.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CASP2 and RIPK1 domain containing adaptor with death domain
• Database Link:
  Entrez Gene 1116 HumanEntrez Gene 8738 Human
  UniProt ID P78560
• Background: CRADD, also referred as RAIDD, is an adaptor protein that could interact with both caspase 2 and RIP that can promote apoptosis once activated. RAIDD (RIP-associated ICH-1/Ced-3 homologous protein with a death domain) has been identified as a RIP binding protein that also associates with members of the caspase family, providing a link between activation of the TNF-Rs and the triggering of the cysteine protease cascade. The amino-terminal domain of RAIDD shares significant homology with the prodomain of ICH-1 and mediates the binding of RAIDD to this cysteine protease.
• Synonyms: CRADD

Reference Data

• Protein Families: Secreted Protein