MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: M2-7C10 + M2-9E3]

CAT#: AM50293PU-T

MelanA (MLANA) mouse monoclonal antibody, clone M2-7C10 + M2-9E3, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded human melanoma stained with Melan-A Antibody (Clone M2-7C10 + M2-9E3). Note cytoplasmic staining of cells.

Specifications

Product Data

• Clone Name: M2-7C10 + M2-9E3
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human MART-1 protein (M2-7C10; M2-9E3).
• Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 20-22 kDa (doublet)
• Gene Name: melan-A
• Database Link:
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Synonyms: Melan-A protein, MLANA, Antigen SK29-AA, LB39-AA, MelanA, MART1