IGKC Mouse Monoclonal Antibody [Clone ID: KLC264]

CAT#: AM50287PU-T

IGKC mouse monoclonal antibody, clone KLC264, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with Kappa Light Chain Antibody (Clone KLC264). Note cell membrane & cytoplasmic staining.

Specifications

Product Data

• Clone Name: KLC264
• Applications: FC, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 min at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
  Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Ig kappa chain.
• Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
  Cellular Localization: Cell Surface, cytoplasmic and secreted.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~22.5 kDa
• Gene Name: immunoglobulin kappa constant
• Database Link:
  Entrez Gene 3514 Human
  UniProt ID P01601
• Synonyms: HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM