Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: K8.8 + DC10]

CAT#: AM50261PU-T

Cytokeratin 8 (KRT8) mouse monoclonal antibody, clone K8.8 + DC10, Purified

Product Images

Formalin-paraffin colon carcinoma stained with Cytokeratin 8/18 Antibody (Clone K8.8+DC10).

Specifications

Product Data

• Clone Name: K8.8 + DC10
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Keratin preparation from a Human carcinoma (K8.8) and PMC-42 Human breast carcinoma cells (DC10).
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18).
  This Monoclonal Antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative.
  Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 52.5 kDa (CK8); 45 kDa (CK18)
• Gene Name: keratin 8
• Database Link:
  Entrez Gene 3856 Human
  UniProt ID P05787
• Synonyms: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18