CD11b (ITGAM) Rat Monoclonal Antibody [Clone ID: M1/70]

CAT#: AM50206PU-T

CD11b (ITGAM) rat monoclonal antibody, clone M1/70, Purified

Specifications

Product Data

• Clone Name: M1/70
• Applications: IF, IHC, IP
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Functional Studies: Use Antibody without BSA and Azide.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Positive Control: Monocytes & granulocytes, Lymph nodes and tonsils.
• Reactivities: Baboon, Chimpanzee, Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: B10 Mouse spleen cells enriched for T lymphocytes.
• Specificity: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 95 kDa & 170 kDa
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
  UniProt ID P11215
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1