CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: CRIS-3]

CAT#: AM50204PU-T

CD11a (ITGAL) mouse monoclonal antibody, clone CRIS-3, Purified

Product Images

FCM staining of Human PBMCs using CD11a / ITGAL Antibody (Clone CRIS-3).

Specifications

Product Data

• Clone Name: CRIS-3
• Applications: FC, IF, IHC, IP
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Functional Studies: Use BSA and Azide free Antibody.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Positive Control: JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Stimulated Human leukocytes.
• Specificity: Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop II; Code N202). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.
  Cellular Localization: Cell surface
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180 kDa
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
  UniProt ID P20701
• Synonyms: Integrin alpha-L, LFA1, LFA-1