Cytokeratin 4+5+6+8+10+13+18 Mouse Monoclonal Antibody [Clone ID: SPM583]

CAT#: AM50198PU-S

Cytokeratin 4+5+6+8+10+13+18 mouse monoclonal antibody, clone SPM583, Purified

Product Images

Formalin-Paraffin Colon (10X) stained with Multi Keratin Antibody Cat.-No AM50198PU (Clone SPM583).

Specifications

Product Data

• Clone Name: SPM583
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µ/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: A431 cells, Skin, Colon carcinoma.
• Reactivities: Bovine, Frog, Guinea Pig, Goat, Human, Marmoset, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Keratin-enriched preparation from cultured Human A431.
• Specificity: This Monoclonal Antibody recognizes Cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: Multiple
• Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Synonyms: Cytokeratin pan-reactive, pan Keratin