Von Willebrand Factor (VWF) Mouse Monoclonal Antibody [Clone ID: SPM577]

CAT#: AM50187PU-T

Von Willebrand Factor (VWF) mouse monoclonal antibody, clone SPM577, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with VWF Antibody (Clone SPM577).

Specifications

Product Data

• Clone Name: SPM577
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: HUVEC cells or Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human vWF fragment.
• Specificity: This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi’s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 250 kDa
• Gene Name: von Willebrand factor
• Database Link:
  Entrez Gene 7450 Human
  UniProt ID P04275
• Background: Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds.
• Synonyms: vWF, von Willebrand antigen 2, von Willebrand antigen II, F8VWF, Factor VIII Related Antigen