Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: SPM576]

CAT#: AM50186PU-S

Vimentin (VIM) mouse monoclonal antibody, clone SPM576, Purified

Product Images

Formalin-paraffin human melanoma stained with Vimentin Antibody (Clone SPM576).

Specifications

Product Data

• Clone Name: SPM576
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells
  Immunofluorescence: 1-2 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat cells, Sarcomas, Melanomas.
• Reactivities: Bovine, Canine, Chicken, Feline, Goat, Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human vimentin recombinant protein.
• Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as Desmin, Keratin, neurofilament, and glial fibrillary acid protein. Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
  Cellular Localization: Cytoplasmic.
  Negative Species: Mouse and Rat.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57-60 kDa
• Database Link:
  Entrez Gene 7431 Human
  UniProt ID P08670
• Synonyms: VIM