BCL2 Mouse Monoclonal Antibody [Clone ID: SPM117]

CAT#: AM50163PU-S

BCL2 mouse monoclonal antibody, clone SPM117, Purified

Product Images

Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody (Clone SPM117). Note nuclear membrane & cytoplasmic staining.

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  Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Antibody (Clone SPM117) (red) and isotype control (green).

Specifications

Product Data

• Clone Name: SPM117
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
• Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein.
  It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
  Cellular Localization: Cytoplasmic & Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25-26 kDa
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Synonyms: BCL2, Bcl-2 alpha