CTLA4 (36-161) Mouse Monoclonal Antibody [Clone ID: AT4F2]

CAT#: AM50045PU-S

CTLA4 (36-161) mouse monoclonal antibody, clone AT4F2, Purified

Product Images

The extract of Mouse Liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT4F2
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot: Recommended starting dilution is 1:3000.
  Immunocytochemistry / Immunoflourescence.
  Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human CTLA4 (36-161aa) purified from E. coli
• Specificity: This antibody detects CTLA4 at aa 36-161.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cytotoxic T-lymphocyte associated protein 4
• Database Link:
  Entrez Gene 1493 Human
  UniProt ID P16410
• Background: CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.
• Synonyms: CTLA-4