GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]
GCLM mouse monoclonal antibody, clone AT2D12, Purified
|Applications||ELISA, FC, IF, WB|
Western blot: 1/3000 (using HeLa cells (40 µg), resolved by SDS-PAGE).
Flow cytometry: 2-5 µg in 1x106 cells (in HeLa cell line).
Immunofluorescence/Immnunocytochemistry: 1/100 (in HeLa cell line).
|Immunogen||Recombinant human GCLM (1-274aa) purified from E. coli|
|Formulation||PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State: Liquid purified Ig fraction
|Purification||Protein-G affinity chromatography|
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1|
|Background||GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.|
|Synonyms||Gamma-ECS regulatory subunit, GCS light chain|
|Protein Families||Druggable Genome|
|Protein Pathways||Glutathione metabolism, Metabolic pathways|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.