GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]

CAT#: AM50016PU-S

GCLM mouse monoclonal antibody, clone AT2D12, Purified

Product Images

Western blot using GCLM / GLCLR antibody no. : The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2D12
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot: 1/3000 (using HeLa cells (40 µg), resolved by SDS-PAGE).
  Flow cytometry: 2-5 µg in 1x10⁶ cells (in HeLa cell line).
  Immunofluorescence/Immnunocytochemistry: 1/100 (in HeLa cell line).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human GCLM (1-274aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1
• Database Link:
  Entrez Gene 2730 Human
  UniProt ID P48507
• Background: GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.
• Synonyms: Gamma-ECS regulatory subunit, GCS light chain

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways