ARMET (MANF) Mouse Monoclonal Antibody [Clone ID: 1G12]

CAT#: AM33442PU-N

ARMET (MANF) mouse monoclonal antibody, clone 1G12, Aff - Purified

Product Images

WesternBlot testing of anti-MANF monoclonal antibody. Lane 1. Page Ruler prestained protein ladder, Fermentas; Lane 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85 cell line.

Specifications

Product Data

• Clone Name: 1G12
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/32000.
  Western Blot: 1/2000-1/4000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human MANF produced using CHO-based.
  Antigen is purified from cell culture supernatant.
• Specificity: Recognizes Human MANF.
  Other species not tested.
• Formulation: PBS pH 7.4
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mesencephalic astrocyte derived neurotrophic factor
• Database Link:
  Entrez Gene 7873 Human
  UniProt ID P55145
• Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
• Synonyms: Arginine-rich protein, MANF